Staybrite dluciferin is a luminescent substrate for firefly luciferase km 2. D luciferin is a chemiluminescent substrate of firefly luciferase. In an immunocompetent mouse model of ovarian cancer, use of dluciferin substrate and firefly luciferase preserves tumorhost immune interactions, since bioluminescent imaging is a more sensitive indication of tumor growth than weight gain. Because chemiluminescent techniques are virtually backgroundfree, this reporter gene system is ideal for detecting lowlevel gene expression. Luciferin amides enable in vivo bioluminescence detection of. Aqueous solutions are usually made up as 25 mm stock solutions 100x in water a1006 or in 25 mm naoh a1029. As with all other luciferins, oxygen is required to elicit light. Apollo scientific home products dluciferin, free acid 99. The water soluble substrate for the firefly luciferase. The study also reported the presence of firefly dluciferin in the glow. Product recommendations pipet sterilizing can, square, 2x2x6. Extensively utilized in reporter gene assays, immunoassays and hygiene monitoring.
It is the substrate for the firefly luciferase enzyme which utilizes atp a. A 1,3thiazolemonocarboxylic acid consisting of 3,5dihydrothiophene4carboxylic acid having a 6hydroxybenzothiazol2yl group at the 2position. Structure, properties, spectra, suppliers and links for. Photinus pyralis luciferase is an enzyme that appears to have no prosthetic groups or tightly bound metal ions and is comprised of 550. Dluciferin can be employed to assay the expression of the luciferase gene linked to a promoter of interest. Dluciferin is the substrate for firefly luciferases bioluminescence reaction, while lluciferin is the substrate for luciferylcoa synthetase activity. Items available from stock in japan will be delivered in 12 weeks.
Synthesis of dluciferin the chemical structure of dluciferin 1, isolated from firefly tails, was proposed in 19618 and. Dluciferin, also known as firefly luciferin, is a lightemitting compound, found in many firefly lampyridae species. Luciferin preparation of luciferin for in vitro and in vivo. This firefly luciferase assay kit is designed for simple and efficient quantitation of firefly luciferase reporter enzyme activity from cultured cells with high sensitivity and linearity. Firefly d luciferin free acid 99% photinus pyralis, pyrophorus, and elateridea substrate luciferinsthis is the free acid and not a water soluble salt, such as the potassium or sodium salts. Luciferin is a common bioluminescent reporter used for in vivo imaging of the expression of luciferase. D luciferin is a popular bioluminescent substrate of luciferase in the presence of atp, used in luciferasebased bioluminescence imaging and cellbased highthroughput screening applications. Dluciferin free acid can be dissolved at a concentration of 10 mgml 35. Molecular formula c11h8n2o3s2, molecular weight 280.
It requires the addition of dilute naoh or koh to aid the free acid to go into solution. Firefly luciferin is a substrate of the abcg2 transporter and has been used as part of a bioluminescence imaging high thoroughput assay to screen for inhibitors of the transporter. Nzdevdaluc was obtained via a hybrid liquidsolid phase synthesis method, in which the appropriately protected. Firefly luciferin, 2591175, ac1ntaep, s26hydroxy2benzothiazolyl2thiazoline4carboxylic acid, s26hydroxybenzo d thiazol2yl4,5dihydrothiazole4. Luciferin preparation of luciferin for in vitro and in. It is a member of benzothiazoles, a 1,3thiazolemonocarboxylic acid and an imidothioate. In an immunocompetent mouse model of ovarian cancer, use of d luciferin substrate and firefly luciferase preserves tumorhost immune interactions, since bioluminescent imaging is a more sensitive indication of tumor growth than weight gain.
Dluciferin, free acid d luciferin is a substrate for the enzyme firefly luciferase. Kg offers a comprehensive product range from building blocks to intermediates and reagents for the chemical and pharmaceutical industry, research institutes and analytical laboratories. Firefly luciferin and modified substrates are fatty acid mimics and have been used to localize fatty. Dluciferin is a popular bioluminescent substrate of luciferase in the presence of atp, used in luciferasebased bioluminescence imaging and cellbased high. Dluciferin is a chemiluminescent substrate of firefly luciferase.
Photinus luciferin is a 1,3thiazolemonocarboxylic acid consisting of 3,5dihydrothiophene4carboxylic acid having a 6hydroxybenzothiazol2yl group at the 2position. Several times freezing and thawing of the stock solution does not influence the quality. Dluciferin free acid 2591175 suppliers buyersguidechem. Thermo scientific pierce firefly luciferase flash assay kit contains reagents to measure the activity of firefly luciferase in mammalian whole cell lysates.
Download certificates of analysis and safety data sheets online. This kit is a new and improved replacement for the firefly luciferase assay kit catalog no. Alternatively, dluciferin and luciferase can be used to assess atp availability in cellular or biochemical assays. Oxyluciferin, a luminescence product of firefly luciferase. Firefly luciferase is best known for its light emission chemistry with dluciferin, but it is also a longchain fatty acylcoa synthetase acsl that can bind fatty acid substrates such as arachidonic acid. Dissolving 10 mgml dluciferin sodium salt only requires a few swirl.
Dluciferin can be utilized to assay the expression of the luciferase gene linked to. Our laboratories execute syntheses in the range of 1 gram. However, the use of glyoxylic acid, either as the free acid or bound to macroporous polystyrene. Apr 19, 2018 a general strategy for the synthesis of npeptide6amino d luciferin conjugates has been developed.
A highly sensitive assay for adenosine triphosphate employing an improved firefly luciferase reagent. Firefly luciferin, 2591175, ac1ntaep, s26hydroxy2benzothiazolyl2thiazoline4carboxylic acid, s26hydroxybenzodthiazol2yl4,5dihydrothiazole4. The substrate for the bioluminescence reaction is d luciferin. Dluciferin, free acid dluciferin is a substrate for the enzyme firefly luciferase. Please note that we cannot offer bulk quantities for some. Pdf the chemiluminescence properties of firefly luciferin were studied, in a variety of chemical systems different from. The biothema dluciferin has a 5 years shelf life from date of delivery at 20 c. Dluciferin bioluminescent substrate buy dluciferin from.
Apr 21, 2016 firefly luciferin, the substrate for the bioluminescence reaction of luminous beetles, possesses a benzothiazole ring, which is rare in nature. This substrate for the firefly luciferase enzyme utilizes atp. Dluciferin free acid 4,5dihydro26hydroxy2benzothiazoyl4thiazolecarboxylic acid, 250 milligrams. Containing a convenient lysis buffer, assay buffer and firefly substrate, this kit provides a complete reagent system for the intracellular detection of promoter or pathway activity. Dluciferin potassium salt is a useful substrate for the enzyme firefly luciferase. Luciferins behave like drugs acs chemical neuroscience. Decarboxylation of lcysteine is a key step for benzothiazole ring formation in firefly luciferin synthesis. Product recommendations benchmark st180 co2 incubator, 180 liter, 115v with three shelves. The applicability of the strategy was demonstrated with the preparation of a known substrate, nzaspgluvalasp6amino d luciferin nzdevdaluc.
Please select the one that is most appropriate for your business. Dluciferin bioluminescent substrate buy dluciferin. D luciferin, also known as firefly luciferin, is a lightemitting compound, found in many firefly lampyridae species. Dluciferin can be utilized to assay the expression of the luciferase gene linked to a promoter of interest. D luciferin free acid for oxidation of luciferin by luciferase produces light, used as a reporter in bacteria, plants, and mammalian cells the atpdependent oxidation of luciferin by luciferase produces light. Dluciferin firefly, free acid cas 2591175 p212121 store. Luciferin as powder and aqueous solutions of luciferin are stable at 20c or 70c longterm storage.
Firefly and renilla luciferase and luciferin have been used for decades to measure atp in preparations. Firefly dluciferin free acid 99% photinus pyralis, pyrophorus, and elateridea substrate luciferinsthis is the free acid and not a water soluble salt, such as the potassium or sodium salts. A benzothaizole which is oxidized by luciferases, firefly to cause emission of light luminescence. This information is stored within a cookie and setting this assumes acceptance. Dluciferin, the substrate for firefly luciferase, gets a gold star for small size, but everything else has room for improvement. Dluciferin free acid for oxidation of luciferin by luciferase produces light, used as a reporter in bacteria, plants, and mammalian cells the atpdependent oxidation of. Luciferin preparation of luciferin for in vitro and in vivo bioluminescent assays preparation of luciferin for in vitro bioluminescent assays materials dluciferin firefly, potassium salt, 1.
Light emission in the american firefly, photinus pyralis, has been shown to involve the interaction of magnesium ion, oxygen, atp the coenzyme adenosine triphosphate, the enzyme luciferase, and the oxidizable substrate luciferin. We recently reported on the biosynthesis of firefly d luciferin, in which the starting materials are lcysteine and 2cyano6hydroxybenzothiazole chbt 1 and the intermediate is l luciferin. We offer the luciferin free acid, as well as its watersoluble sodium and potassium salts l2912, l2916. Onepot nonenzymatic formation of firefly luciferin in a. D luciferin free acid d luciferin free acid, minimum 99% by achiral hplc and minimum 99. Here, we synthesized luciferin amides and found that these molecules are hydrolyzed to substrates for firefly luciferase by the enzyme. Firefly luciferin is the luciferin, or lightemitting compound, used for the firefly lampyridae. We offer the luciferin free acid l2911, as well as its watersoluble sodium and potassium salts. Dluciferin is a substrate for the enzyme firefly luciferase. Downloaded by universidade do porto up on 300520 12.
Lluciferin and dluciferin inhibit the bioluminescence pathway and the coaligase pathway, respectively. Chemiluminescence at 560 nm is generated when luciferin is oxidized by an atpdependent process, catalyzed by luciferase. Firefly luciferin, which is identical to beetle luciferin, is a prominent bioluminescent substrate. This requires reconsititution using alkaline buffers. Pdf biosynthesis of firefly luciferin in adult lantern. Synthesis of dluciferin the chemical structure of dluciferin 1, isolated from firefly tails, was proposed in 19618 and later confirmed by synthesis. Explore all research articles, conference papers, preprints and. The atpdependent oxidation of luciferin by luciferase produces light, and this system is. Dluciferin is a substrate for firefly luciferase with a km of approx 2. D luciferin is commonly used as a substrate for imaging the expression of the luc marker gene as well as luciferase fusion conjugates in living cells, tissues and organisms. Dluciferin is a common bioluminescent reporter used for in vivo imaging of the expression of luciferase. Upon oxidation by the enzyme luciferase, produces bioluminescence. The substrate for the bioluminescence reaction is dluciferin. Recent work has shown that the enzyme firefly luciferase will accept many substrate analogues, allowing tuning of the molecular.
Apollo scientific currently provides pricing in several currencies. S26hydroxy2benzothiazolyl2 thiazoline4carboxylic acid. Dluciferin firefly, free acid proven and published. Our dmnpecaged d luciferin l7085 offers an efficient way to deliver luciferin into intact cells and can be used to supply a continuous source of active luciferin. Trishydroxymethylaminomethane tris base or sodiumpotassium bicarbonate. Firefly luciferase is homologous to fatty acylcoa synthetases. A general strategy for the synthesis of npeptide6aminodluciferin conjugates has been developed. Dluciferin sodium salt cas 103404757 cayman chemical. Dluciferin firefly, free acid proven and published zellbio gmbh. Firefly luciferin, the substrate for the bioluminescence reaction of luminous beetles, possesses a benzothiazole ring, which is rare in nature. The firefly luciferinluciferase reaction is a bioluminescence reaction. Dluciferin cas 2591175 scbt santa cruz biotechnology. We hypothesized that the firefly luciferase substrate d luciferin and its analogs are fatty acid mimics that are ideally suited to probe the chemistry of enzymes that release fatty acid products. D luciferin potassium salt is a useful substrate for the enzyme firefly luciferase.
Download product insert pdfdownload safety data sheet sds pdf. Pdf firefly luciferin as a multifunctional chemiluminescence. Used with firefly luciferase in bioluminescence for the determination of atp. D luciferin is a substrate for the enzyme firefly luciferase. Nzdevdaluc was obtained via a hybrid liquidsolid phase synthesis method, in which the appropriately protected cterminal amino acid was. S26hydroxy2benzothiazolyl2thiazoline4carboxylic acid. Dluciferin free acid dluciferin free acid, minimum 99% by achiral hplc and minimum 99.
Luciferin is a generic name given to the class of small organic molecules that can emit light upon oxidation catalyzed by various types of luciferases, among which firefly luciferin and coelenterazine are the most widely used in biotechnology and, recently, in in vivo imaging. Dluciferin firefly, free acid proven and published goldbio. Buy dluciferin cas 2591175, a synthetic substrate for firefly luciferase, from santa cruz. Both reactions are inhibited by the substrates enantiomer. Dluciferin free acid 4,5dihydro26hydroxy2 benzothiazoyl 4thiazolecarboxylic acid, 1 gram. It is a member of benzothiazoles, a 1,3thiazolemonocarboxylic acid and. D luciferin can be utilized to assay the expression of the luciferase gene linked to a promoter of interest. Dluciferin is commonly used as a substrate for imaging the expression of the luc marker gene as well as luciferase fusion conjugates in living cells, tissues and organisms. Fireflies produce light by the oxidation of dluciferin to an excitedstate molecule. Staybrite d luciferin is a luminescent substrate for firefly luciferase km 2.
The free acid form of luciferin requires addition of a dilute base such as naoh or koh to dissolve in water. The applicability of the strategy was demonstrated with the preparation of a known substrate, nzaspgluvalasp6aminodluciferin nzdevdaluc. Firefly luciferase requires atp for its reaction, emitting a greenishyellow luminescence at a peak wavelength of approximately 530 nm. Because of its specific light emission with high quantum yield, the bioluminescence signal is rapidly detected using conventional light detectors and the reaction is used widely as a convenient analytical tool in life science research and industry. Biosynthesis of firefly luciferin in adult lantern. Firefly luciferin and modified substrates are fatty acid mimics and have been used to localize fatty acid amide hydrolase faah in vivo. Predicted data is generated using the acdlabs percepta. Conversely, we have recently shown that an acsl from the fruit fly drosophila melanogaster is a latent luciferase that can emit light with a synthetic luciferin. Luciferin amides enable in vivo bioluminescence detection. Our dmnpecaged dluciferin l7085 offers an efficient way to deliver luciferin into intact cells and can be used to supply a continuous source of active luciferin.
1532 1583 1651 275 1526 912 129 1505 963 31 1416 20 961 858 1226 747 1141 1278 381 1112 105 92 400 1616 372 18 504 860 119 909 1664 915 986 309 986 771 695 556 694 500